The spatiotemporal characteristics of the intraventricular flow field, and their evolution with time, are of (patho)physiological and clinical interest. This is shown in several recent in vivo studies, using modern medical imaging techniques. Intraventricular flow is also topic of biofluid mechanic research, in silico and in vitro. Particle image velocimetry (PIV) studies have been performed in hydraulic bench models to investigate the flow field inside a left ventricle (LV) replica in a two-dimensions. However, as the intraventricular flow has a complex 3D and unsteady structure. In this study therefore we present a novel experimental setup which allows 3D volume reconstruction of the flow field in a transparent pulsatile LV membrane model, in different controllable and repeatable physiological relevant hydrodynamic conditions. The setup was primarily designed and developed to facilitate consecutive stereoscopic measurements without repeating the complex and time consuming stereo calibration.